Review



dh dopamine hydrochloride standard sigma  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    R&D Systems dh dopamine hydrochloride standard sigma
    Dh Dopamine Hydrochloride Standard Sigma, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dh+dopamine+hydrochloride+standard+sigma/Recombinant+Human+G6PD+His-tag+Protein%2C+CF/pm39772392-226-17-14
    Average 93 stars, based on 2 article reviews
    dh dopamine hydrochloride standard sigma - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Plasmid Preparation:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Transfection:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Virus:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Quantitative RT-PCR:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Titration:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Reverse Transcription:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    SYBR Green Assay:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    DC Protein Assay:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Activity Assay:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome

    Metabolomic:

    Article Title: G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Urea Fisher Cat# PI29700 Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagentsTM Fisher Cat# BP166 NaF/Sodium Fluoride (Powder/Certified ACS), Fisher ChemicalTM Fisher Cat#S299100 PMSF/Phenylmethanesulfonyl fluoride R98.5% (GC) Sigma Cat# P7626 NaV/Sodium orthovanadate, 99% Fisher/ACROS Organics Cat# 205330500 Aprotinin Bioshop APR600.25 Sucrose/D-Sucrose (Molecular Biology), Fisher BioReagents Fisher Cat# FLBP220212 Imidazole/Imidazole (Molecular Biology), Fisher BioReagentsTM Fisher Cat# BP305-50 EDTA/Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate (Crystalline/Certified ACS), Fisher ChemicalTM Fisher Cat# S311 Cycloheximide/Cycloheximide, High Purity - CAS 66-81-9 - Calbiochem Sigma Cat#239764 Clarity Western ECL Substrate Bio-Rad Cat# 1705061 IPTG/Isopropyl b-D-1-thiogalactopyranoside Bioshop Cat# IPT002.25 LB media Sigma Cat#L3522 Ampicilline, Sodium Salt - CAS 69-52-3 - Calbiochem Sigma Cat# 171254 5X Reducing Sample Buffer/Thermo ScientificTM PierceTM Lane Marker Reducing Sample Buffer Fisher Cat#PI39000 mTeSRTM PlusComplete Kit STEMCELL Technologies Inc Cat# 100-0276 ReLeSRTM passaging reagent STEMCELL Technologies Inc Cat# 100-0484 Accutase solution Sigma Cat# A6964 Matrigel/CorningTM MatrigelTM hESC-Qualified Matrix Fisher/Corning Cat# 08-774-552 KSR/GibcoTM KnockOutTM Serum Replacement Fisher/Gibco Cat#10828010 B27/GibcoTM B-27TM Supplement (50X), serum free Fisher/Gibco Cat#17504044 GibcoTM N-2 Supplement (100X) Fisher/Gibco Cat# 17502048 DMEM/Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture Sigma Cat#D8437 Gibco Antibiotic-Antimycotic (100X) Fisher Cat# 15240062 Gibco Bovine Albumin Fraction V (7.5% solution) Fisher Cat# 15260037 Gibco b-mercaptoethanol Fisher Cat# 21985023 Fetal Bovine Serum (FBS)/FBS Optima R&D Systems Cat# S12450 HyCloneTM Non-Essential Amino Acids (NEAA) 100X Fisher Cat# SH3023801 Gibco Penicillin-Streptomycin (10,000 U/mL)/Pen Strep Fisher Cat# 15-140-122 Gibco 100mM Sodium Pyruvate Solution Fisher Cat# 11360070 (Continued on next page) 18 Cell Reports 44, 115178, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gelatin/Gelatin from bovine skin Sigma Cat# G9391 G6PD Recombinant R&D Systems Cat# 10096-DH Dopamine hydrochloride Standard Sigma Cat# 1225204 Doxycycline hyclate Sigma Cat# D9891 HiSpeed Maxi Plasmid kit Qiagen Cat# 12662 Opti-MEM Fisher Cat# 31985-062 Polyethyleneimine (PEI transfection reagent) Santacruz Cat# sc-507213 Polyethylene Glycol 8000 (PEG), Fisher BioReagents Fisher BP233-1 Nucleospin RNA Virus Takara Bio Cat# 740956.10 Lenti-X qRT-PCR Titration Kit Takara Bio Cat# 631235 Quantitect Reverse Transcription kit Qiagen Cat# 205311 SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Dynabeads protein G Invitrogen Cat#10004D Critical Commercial Assays Bio-Rad DC Protein assay Bio-Rad Cat#5000112 G6PD activity assay Abcam Cat# ab176722 Glutathione Colorimetric Detection Kit Invitrogen Cat# EIAGSHC Fluorometric NADP/NADPH Assay Kit Abcam Cat# ab176724 Deposited Data Metabolomic Data Metabolights Metabolights:MTBLS11250 Experimental Models: Cell Lines Human: hESC-derived WT and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: hiPSC-derived Corrected and A53T cell lines Gifted from Rudolf Jaenisch, Whitehead Inst, MIT, Boston, MA N/A Human: SHSY5Y cells ATCC Cat# CRL-2266 Experimental models: Organisms/strains Mouse: B6C3F1/J The Jackson Laboratory Jax: 100010 Mouse: B6; C3-Tg(Prnp-SNCA*A53T)83Vle/J The Jackson Laboratory Jax: 004479 Oligonucleotides G6PD forward Sigma AGCTGGAGGACTTCTTTGCC G6PD reverse Sigma TGATGCGGTTCCAGCCTATC Recombinant DNA pET21a-alpha-synuclein Addgene Cat#51486 pET21a-alpha-synuclein-delta-C-Term (a.a. 1–119) Genscript Custom pET28a+-G6PD-Sumo Genscript Custom pWPXLd_EF1a_Trx1_IRES_EGFP Genscript Custom pWPXLd backbone Addgene 12258 pLenti6.2_mCherry_Grx1_roGFP2 Addgene 155045 shScramble-GFP Origene TR30021 shG6PD-A-GFP Origene TL312877A - ATGAGCCAGATAGGCTGG AACCGCATCAT shG6PD-D-GFP Origene TL312877D - CAGCCGTCGTCCTCTATG TGGAGAATGAG (Continued on next page) Cell Reports 44, 115178, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms Axio-observer Zen Pro software Zeiss https://www.zeiss.com/microscopy/int/ products/microscope-software/zen.html ImageJ National Institute of Health https://imagej.nih.gov/ij/download.html Image Studio Software For Li-Cor Odyssey Fc LI-COR https://www.licor.com/bio/image-studio/ FPLC DuoLogic system Bio-Rad N/A Discontinued system HPLC Waters system Millennium 32 software Waters N/A Prism7 Graphpad Software Inc https://www.graphpad.com/scientific- software/prism/ MAVEN Open-source http://maven.princeton.edu NanoAnalyze (V3.0) TA Instruments https://www.tainstruments.com/sw/ nano_analyze.html AlphaFold3 DeepMind https://alphafoldserver.com/welcome



    Similar Products

    dh dopamine hydrochloride standard sigma  (R&D Systems)
    93
    R&D Systems dh dopamine hydrochloride standard sigma
    Dh Dopamine Hydrochloride Standard Sigma, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dh+dopamine+hydrochloride+standard+sigma/Recombinant+Human+G6PD+His-tag+Protein%2C+CF/pm39772392-226-17-14
    Average 93 stars, based on 1 article reviews
    dh dopamine hydrochloride standard sigma - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results